Part:BBa_K3781100:Design
pAGM1276
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 1640
Illegal XbaI site found at 1613
Illegal PstI site found at 1601 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 1640
Illegal PstI site found at 1601 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 1640
Illegal BamHI site found at 1619 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 1640
Illegal XbaI site found at 1613
Illegal PstI site found at 1601 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 1640
Illegal XbaI site found at 1613
Illegal PstI site found at 1601
Illegal NgoMIV site found at 299 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 1388
Illegal BsaI.rc site found at 1998
Illegal SapI site found at 2059
Illegal SapI.rc site found at 1375
Design Notes
This plasmid backbone was not designed by us, yet simply utilized from the Modular Cloning Toolbox established by Weber et al.[1]. We purchased it from our supervising working group that is working with MoClo in Chlamydomonas reinhardtii. Despite this part not being thought up, engineered or modified by us, we decided to include it into the registry in order to give possible users of our MocloMania collection a holistic view on the genetic material needed to build their own MoClo library and carry out their desired MoClo assemblies.
Source
The plasmid's sequence is directly derived from the creators of the Weber et al. Modular Cloning Kit. It can be found online as the Addgene plasmid named pAGM1276, #47986. The basic make-up of the plasmid is an alteration of the standard pUC19/pUC18 plasmids that have been a common lab staple for cloning in E. Coli for decades.[2] The sequences included within the plasmid, such as the N-terminal beta-galactosidase fragment lacZ-alpha and the ori are modifications of genes found within the genome of many E. Coli strains.
References
- ↑ Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S (2011) A Modular Cloning System for Standardized Assembly of Multigene Constructs. PLoS ONE 6(2): e16765. https://doi.org/10.1371/journal.pone.0016765
- ↑ C. Yanisch-Perron, J. Vieira, J. Messing: Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. In: Gene. Band 33, Nummer 1, 1985, S. 103–119. PMID 2985470.